First publication on the concept of masscytometry

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how many CyTOFs are there in Germany

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all participants of the last 3 meetings

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Getting started: tutorial

Getting started with mass cytometry and imaging mass cytometry. How does it work, what to expect, how and when to leverage its strength. All you need to know about setting up the platform, assay design and basic workflows.

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Getting started: tutorial

News from…

The growing number of German Mass Cytometry Centers have their place here. Centers can present themselves, commonly with 1-2 projects selected by the local teams.

And again Beckman Coulter/Cytobank donates a year's licence of the Cytobank Premium SW for the best Speed Talk at the 4th German Mass Cytometry User Forum!

News from…

PROGRAM

Please note: The presentations will not be recorded - so be there live!
We will close the registration on 19.2.2021 at 12.00 noon!

  • Day 1 21. Januar 2021
  • Day 2 22. Januar 2021
  • Zoom
getting started button
10:30 - 12:15Getting started… By Desiree Kunkel, Axel Schulz, Henrike Salié, Bertram Bengsch, Sarah Warth

…with mass cytometry

10:30Welcome by Henrik Mei
10:45Désirée Kunkel, Berlin: Principles of mass cytometry (10)
10:55Axel Schulz, Berlin: Suspension mass cytometry – basic workflows (20)
11:15Désirée Kunkel, Berlin: Introduction to Imaging mass cytometry (10)
11:25Henrike Salié/Bertram Bengsch, Freiburg: Imaging mass cytometry – basic workflows (20)
11:45Sarah Warth, Ulm, and all presenters: Q&A, tips and tricks (30) please send questions to axel.schulz@drfz.de
12:15Break (65)

13:20 - 14:30Session 1 – Mass Cytometry vs COVID-19Chairs: Bertram Bengsch & Henrik Mei

Chairs: Bertram Bengsch & Henrik Mei

13:20Birgit Sawitzki, Berlin: CyTOF-mediated characterisation of the myeloid cell compartment in severe COVID-19 (15+5)
13:40Henrike Salie, Freiburg: Spatial single-cell mapping reveals an altered local immune response in COVID-19 brains (15+5)
14:00Short talk: Malte Lehmann, Berlin: Human small intestinal infection by SARS-CoV2 is characterized by an activation of CD8+ T cells (12+3)
14:15Short talk: Dena Panovska, Leuven: Mapping the recovery of critically ill COVID19 patients by high-dimensional profiling identifies blood immunotypes following a specific immune trajectory(12+3)
14:30Coffee break (30)
15:00 - 16:20Session 2 News from … & selected abstractsChairs: Marie Burns & Sarah Warth

Chairs: Marie Burns & Sarah Warth

15:00…BIH Berlin: Chotima Böttcher – The uses and limitations of single-cell mass cytometry for studying human microglia function (15+5)
15:20… Ulm: Habib Rahimi: Characterization of acute erythroid leukemia using mass cytometry (15+5)
15.40… MPIMG Berlin: Marie-Laure Yaspo – Interpreting bulk RNAseq with single cell technologies (15+5)
16.00Short talk: Florian Ingelfinger, Zurich: Single-cell profiling of Myasthenia Gravis identifies a pathogenic T cell signature (12+3)
16:20 - 16:40Webinar by OMIQChair: Henrik Mei

Chair: Henrik Mei

16:20 Chris Ciccolella, Santa Clara, USA: Mastering High-Dimensional Analysis with the OMIQ Platform (15+5)
16:40 Coffee break

17:00 - 17:40Session 3Chair: Henrik Mei

Chair: Henrik Mei

17:00Chris Tape, London, UK
Single-Cell Signalling Analysis of Tumour Microenvironment Organoids

  • Zoom
speed talks
10:00 - 11:45Speed talksChairs: Axel Schulz, Desiree Kunkel & Sarah Warth

Chairs: Axel Schulz, Désirée Kunkel & Sarah Warth

10.00 – 11.45Speed talks from selected abstracts (3 min each)
12:00 - 12:40Webinar by Fluidigm By FluidigmChair: Henrik Mei

Chair: Henrik Mei

Speaker: Andrew Quong
Chief Scientific Officer Fluidigm Corporation

Preliminary Title: Mass Cytometry – a glance into the future

Content: Impact of cytometry in infectious disease, cancer and immunology and how Fludigm will be enabling rapid growth in scientific discovery.

Fluidigm

Join our Webinar

January 22, 2021, 12:00 -12:40

13:00 - 14:20Session 4 News from…Chair: Marie Burns

Chair: Marie Burns

13:00…DRFZ: Axel Schulz: Severe COVID‐19 is characterized by an increased induction of peripheral plasmablasts with an aberrant CD62L+HLA‐DRlow phenotype (15+5)
13:20 …Munich: Selina Keppler/Marc Rosenbaum: Establishing CyTOF panels for the analysis of murine immune cells (15+5)
13:40…Freiburg: Lena Sophie Mayer: Alterations in tissue-resident memory and exhausted-like CD8+ T cells in active UC. (15+5)
14.00 ….Granada: Paulina Rybakowska: Experimental and data processing workflow for large-scale immune monitoring studies by mass cytometry (15+5)
14:20Coffee break (20)
14:40 - 16:00Session 5 Data AnalysisChairs: Henrik Mei, Axel Schulz

Chairs: Henrik Mei, Axel Schulz

14:40Lucie Rodriguez, Scilifelab, Stockholm, SE: Multi OMICs data integration (15+5)
15:00Denis Schapiro, Harvard, USA: „Google Maps” for tissue biology – Mapping the tumor microenvironment with spatial omics technologies (25+5)
15:30Mark Robinson: Differential discovery for CyTOF experiments, Univ. Zurich, CH: tba (25+5)

16:15 - 16:35Webinar by Beckman Coulter/Cytobank By Beckmann CoulterChair: Henrik Mei

Join our Webinar

January 22, 2021, 16:15 -16:35

Chair: Henrik Mei

Speaker:
Dr. Giulia Grazia, Beckman Coulter Life Sciences, Italy

Title: Navigate safely through a sea of data: management and analysis made smart!

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16:45 - 17:45Virtual round tableChair: Henrik Mei

Chair: Henrik Mei

16:45 Selected speakers – Discussion of hot topics, Q&A
– Denis Shapiro
– Chris Tape
– Chotima Böttcher
17:45Fare well and good by

Download the abstract book incl. programme overview and list of participants

Who’s Speaking

Here we present our speakers for the Mass Cytometry User Forum 2021

Desiree Kunkel
Désirée KunkelCharité - Universitätsmedizin Berlin & Berlin Institute of Health (BIH)

January 21, 2021, 10.45

Desiree Kunkel
Désirée KunkelCharité - Universitätsmedizin Berlin & Berlin Institute of Health (BIH)

January 21, 2021, 10.45

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Axel Schulz
Axel SchulzGerman Rheumatism Research Center Berlin (DRFZ), a Leibniz Institute

January 21, 2021, 10.45

Axel Schulz
Axel SchulzGerman Rheumatism Research Center Berlin (DRFZ), a Leibniz Institute

January 21, 2021, 10.45

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Bertram Bengsch
Bertram BengschUniversity Medical Center Freiburg

January 21, 2021, 10.45

Bertram Bengsch
Bertram BengschUniversity Medical Center Freiburg

January 21, 2021, 10.45

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Sarah Warth
Sarah WarthUniversity Medical Faculty Ulm

January 21, 2021, 10.45

Sarah Warth
Sarah WarthUniversity Medical Faculty Ulm

January 21, 2021, 10.45

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Birgit Sawitzki
Birgit SawitzkiCharité – Universitätsmedizin Berlin

Talk, January 21, 2021, 13.20

Birgit Sawitzki
Birgit SawitzkiCharité – Universitätsmedizin Berlin

Talk, January 21, 2021, 13.20

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Foto von Henrike Salié
Henrike SaliéUniversity Medical Center Freiburg

Talk: January 21, 2020, 13.40

Foto von Henrike Salié
Henrike SaliéUniversity Medical Center Freiburg

Talk: January 21, 2020, 13.40

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Malte Lehmann
Malte LehmannMalte Lehmann, Division of Gastroenterology, Charité – Universitätsmedizin Berlin

Short Talk, January, 21st, 14.00

Malte Lehmann
Malte LehmannMalte Lehmann, Division of Gastroenterology, Charité – Universitätsmedizin Berlin

Short Talk, January, 21st, 14.00

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Dena Panovska
Dena PanovskaDena Panovska, Deparment of Imaging and Pathology, KU Leuven, Belgium

Short Talk, January, 21st, 14.15

Dena Panovska
Dena PanovskaDena Panovska, Deparment of Imaging and Pathology, KU Leuven, Belgium

Short Talk, January, 21st, 14.15

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Chotima Böttcher
Chotima Böttcher

Talk, January 21, 2020, 15.00

Chotima Böttcher
Chotima Böttcher

Talk, January 21, 2020, 15.00

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Habib Rahimi
Habib RahimiInstitute of Experimental Cancer Research University Hospital Ulm

Talk, January 21, 2021, 15.20

Habib Rahimi
Habib RahimiInstitute of Experimental Cancer Research University Hospital Ulm

Talk, January 21, 2021, 15.20

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Florian Ingelfinger
Florian IngelfingerFlorian Ingelfinger, Institute of Experimental Immunology, University of Zurich, Switzerland

Short Talk, January 21st, 16.00

Florian Ingelfinger
Florian IngelfingerFlorian Ingelfinger, Institute of Experimental Immunology, University of Zurich, Switzerland

Short Talk, January 21st, 16.00

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Chris Ciccolella
Chris CiccolellaCo-Founder & CEO, Omiq, Inc.

Webinar by OMIQ, January 21, 20221, 16.20

Chris Ciccolella
Chris CiccolellaCo-Founder & CEO, Omiq, Inc.

Webinar by OMIQ, January 21, 20221, 16.20

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Chris Tape
Chris TapeUniversity College London Cancer Institute

Talk: January 21, 2021, 17.00

Chris Tape
Chris TapeUniversity College London Cancer Institute

Talk: January 21, 2021, 17.00

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Selina Keppler and Marc Rosenbaum
Selina Keppler and Marc RosenbaumMRI and TranslaTUM, Technical University Munich

Talk, January 22nd, 2021, 13.20

Selina Keppler and Marc Rosenbaum
Selina Keppler and Marc RosenbaumMRI and TranslaTUM, Technical University Munich

Talk, January 22nd, 2021, 13.20

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Lena Sophie Mayer
Lena Sophie MayerUniversitätsklinikum Freiburg

Talk, January 22nd, 2021, 13:40

Lena Sophie Mayer
Lena Sophie MayerUniversitätsklinikum Freiburg

Talk, January 22nd, 2021, 13:40

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Paulina Rybakowska
Paulina RybakowskaPaulina Rybakowska, GENYO, Granada, Spain.

Talk, January 22, 2021, 14.00

Paulina Rybakowska
Paulina RybakowskaPaulina Rybakowska, GENYO, Granada, Spain.

Talk, January 22, 2021, 14.00

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Lucie Rodriguez
Lucie RodriguezScilifelab, Stockholm

Talk, January 22, 2020, 14.40

Lucie Rodriguez
Lucie RodriguezScilifelab, Stockholm

Talk, January 22, 2020, 14.40

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Denis Schapiro
Denis SchapiroHarvard Medical School & Broad Institute of MIT, USA

Talk, January 22, 2021, 15.00

Denis Schapiro
Denis SchapiroHarvard Medical School & Broad Institute of MIT, USA

Talk, January 22, 2021, 15.00

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Mark D RobinsonMark D Robinson, Statistical Bioinformatics, University Zurich, Switzerland

Talk, January 22, 2021, 15.30

Mark D RobinsonMark D Robinson, Statistical Bioinformatics, University Zurich, Switzerland

Talk, January 22, 2021, 15.30

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FAQ’s

Commonly asked questions about the User Forum