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Join our Webinar January 22, 2021, 16:15 -16:35 Chair: Henrik Mei Speaker:Dr. Giulia Grazia, Beckman Coulter Life Sciences, Italy Title: Navigate safely through a sea of data: management and analysis made smart! more details

Chair: Henrik Mei 16:45  Selected speakers – Discussion of hot topics, Q&A– Denis Shapiro– Chris Tape– Chotima Böttcher 17:45 Fare well and good by

Chairs: Henrik Mei, Axel Schulz 14:40 Lucie Rodriguez, Scilifelab, Stockholm, SE: Multi OMICs data integration (15+5) 15:00 Denis Schapiro, Harvard, USA: „Google Maps” for tissue biology – Mapping the tumor microenvironment with spatial omics technologies (25+5) 15:30 Mark Robinson: Differential discovery for CyTOF experiments, Univ. Zurich, CH: tba (25+5)

Chair: Marie Burns 13:00 …DRFZ: Axel Schulz: Severe COVID‐19 is characterized by an increased induction of peripheral plasmablasts with an aberrant CD62L+HLA‐DRlow phenotype (15+5) 13:20  …Munich: Selina Keppler/Marc Rosenbaum: Establishing CyTOF panels for the analysis of murine immune cells (15+5) 13:40 …Freiburg: Lena Sophie Mayer: Alterations in tissue-resident memory and exhausted-like CD8+ T cells in …

Chairs: Axel Schulz, Désirée Kunkel & Sarah Warth 10.00 – 11.45 Speed talks from selected abstracts (3 min each)

Chair: Henrik Mei 17:00 Chris Tape, London, UKSingle-Cell Signalling Analysis of Tumour Microenvironment Organoids

Chair: Henrik Mei 16:20 Chris Ciccolella, Santa Clara, USA: Mastering High-Dimensional Analysis with the OMIQ Platform (15+5) 16:40 Coffee break

Chairs: Marie Burns & Sarah Warth 15:00 …BIH Berlin: Chotima Böttcher – The uses and limitations of single-cell mass cytometry for studying human microglia function (15+5) 15:20 … Ulm: Habib Rahimi: Characterization of acute erythroid leukemia using mass cytometry (15+5) 15.40 … MPIMG Berlin: Marie-Laure Yaspo – Interpreting bulk RNAseq with single cell technologies (15+5) …

…with mass cytometry 10:30 Welcome by Henrik Mei 10:45 Désirée Kunkel, Berlin: Principles of mass cytometry (10) 10:55 Axel Schulz, Berlin: Suspension mass cytometry – basic workflows (20) 11:15 Désirée Kunkel, Berlin: Introduction to Imaging mass cytometry (10) 11:25 Henrike Salié/Bertram Bengsch, Freiburg: Imaging mass cytometry – basic workflows (20) 11:45 Sarah Warth, Ulm, and …

Immune profiling & monitoring are commonly applied in translational and clinical research settings to provide phenotypic understanding of immune states prior to and following treatment (e.g. COVID-19). Mass cytometry, which utilizes CyTOF(r) technology, is a single-cell analysis platform that uses metal-tagged antibodies and can resolve over 50 markers in a single sample tube. We have developed a sample-to-report solution for human immune profiling using mass cytometry, the Maxpar(r) Direct(tm) Immune Profiling System.