with mass cytometry
Abstract
Our introduction to mass cytometry ensures that everyone is at the same level when talking about this technology. Five experts from the field tell you how mass cytometry works and how it can be used to examine cell suspensions and tissue sections. We will guide you through typical experimental workflows and share our experience with important aspects in the application of mass cytometry, such as metal conjugation, sample barcoding, spillover compensation and batch normalization. You will also learn about the advantages of Imaging Mass Cytometry (IMC) and how to establish a multiplexed antibody panel for it. This is complemented by an introduction to current concepts of data analysis, both for imaging and suspension mass cytometry. Following the introductory talks there will also be time to discuss individual questions concerning mass cytometry and its application.
Biosketch Désirée Kunkel
Désirée Kunkel is head of Flow & Mass Cytometry Core Facility of the Berlin Institute of Health (BIH) for 11 years. As one of the first CyTOF operators in Germany, she and her core facility provide professional access to suspension mass cytometry since 2014 and IMC since 2018.
Biosketch Axel Schulz
Axel Schulz joined the DRFZ mass cytometry laboratory in 2014 after gaining first experience with the CyTOF technology at the HIMC in Stanford in 2012. As a postdoc, he works on the technical implementation of the CyTOF technology in various projects and is currently the operator in charge of the institute’s Helios instrument.
Biosketch Henrike Salié
Henrike Salié is performing her doctorate studies in the lab of Prof. Bengsch in the Clinic for Internal Medicine II at the University Medical Center Freiburg. Her research focus is on understanding immune responses to malign, autoimmune and viral challenges in tissues, with a focus on the spatial interactions of exhausted T cells, for which she established and applied an imaging mass cytometry approach.
Biosketch Bertram Bengsch
Bertram Bengsch is Professor for Translational Hepatogastroenterology and head of the Mass Cytometry Facility at the Clinic for Internal Medicine II, University Medical Center Freiburg. He has worked with mass cytometry starting in 2014 at the University of Pennsylvania.
Biosketch Sarah Warth
Sarah Warth coordinates the Core Facility Cytometry in Ulm since 2017. She was formerly working in the core of Désirée Kunkel and where she gained a lot of experience in suspension mass cytometry especially in its application in immunology. The core in Ulm is using a Helios instrument.
Affiliations
- Désirée Kunkel, Flow & Mass Cytometry Core Facility, Charité – Universitätsmedizin Berlin & Berlin Institute of Health (BIH)
- Axel Schulz, Mass Cytometry Lab, German Rheumatism Research Center Berlin (DRFZ), a Leibniz Institute
- Henrike Salié, Bengsch Lab, Clinic for Internal Medicine II, University Medical Center Freiburg
- Bertram Bengsch, Bengsch Lab Clinic for Internal Medicine II, University Medical Center Freiburg
- Sarah Warth, Core Facility Cytometry, Ulm University Medical Faculty
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