Program

Please note: The presentations will not be recorded – so be there live!
Registration is open until 19 January 2021!

10:30 - 12:15Getting started… By Desiree Kunkel, Axel Schulz, Henrike Salié, Bertram Bengsch, Sarah Warth

…with mass cytometry

10:30	Welcome by Henrik Mei
10:45	Désirée Kunkel, Berlin: Principles of mass cytometry (10)
10:55	Axel Schulz, Berlin: Suspension mass cytometry – basic workflows (20)
11:15	Désirée Kunkel, Berlin: Introduction to Imaging mass cytometry (10)
11:25	Henrike Salié/Bertram Bengsch, Freiburg: Imaging mass cytometry – basic workflows (20)
11:45	Sarah Warth, Ulm, and all presenters: Q&A, tips and tricks (30) please send questions to axel.schulz@drfz.de
12:15	Break (65)

13:20 - 14:30Session 1 – Mass Cytometry vs COVID-19Chairs: Bertram Bengsch & Henrik Mei

Chairs: Bertram Bengsch & Henrik Mei

13:20	Birgit Sawitzki, Berlin: CyTOF-mediated characterisation of the myeloid cell compartment in severe COVID-19 (15+5)
13:40	Henrike Salie, Freiburg: Spatial single-cell mapping reveals an altered local immune response in COVID-19 brains (15+5)
14:00	Short talk: Malte Lehmann, Berlin: Human small intestinal infection by SARS-CoV2 is characterized by an activation of CD8+ T cells (12+3)
14:15	Short talk: Dena Panovska, Leuven: Mapping the recovery of critically ill COVID19 patients by high-dimensional profiling identifies blood immunotypes following a specific immune trajectory(12+3)
14:30	Coffee break (30)

15:00 - 16:20Session 2 News from … & selected abstractsChairs: Marie Burns & Sarah Warth

Chairs: Marie Burns & Sarah Warth

15:00	…BIH Berlin: Chotima Böttcher – The uses and limitations of single-cell mass cytometry for studying human microglia function (15+5)
15:20	… Ulm: Habib Rahimi: Characterization of acute erythroid leukemia using mass cytometry (15+5)
15.40	… MPIMG Berlin: Marie-Laure Yaspo – Interpreting bulk RNAseq with single cell technologies (15+5)
16.00	Short talk: Florian Ingelfinger, Zurich: Single-cell profiling of Myasthenia Gravis identifies a pathogenic T cell signature (12+3)

16:20 - 16:40Webinar by OMIQChair: Henrik Mei

Chair: Henrik Mei

16:20	Chris Ciccolella, Santa Clara, USA: Mastering High-Dimensional Analysis with the OMIQ Platform (15+5)
16:40	Coffee break

17:00 - 17:40Session 3Chair: Henrik Mei

Chair: Henrik Mei

17:00

Chris Tape, London, UK
Single-Cell Signalling Analysis of Tumour Microenvironment Organoids

10:00 - 11:45Speed talksChairs: Axel Schulz, Desiree Kunkel & Sarah Warth

Chairs: Axel Schulz, Désirée Kunkel & Sarah Warth

10.00 – 11.45	Speed talks from selected abstracts (3 min each)

12:00 - 12:40Webinar by Fluidigm By FluidigmChair: Henrik Mei

Chair: Henrik Mei

Speaker: Andrew Quong
Chief Scientific Officer Fluidigm Corporation

Preliminary Title: Mass Cytometry – a glance into the future

Content: Impact of cytometry in infectious disease, cancer and immunology and how Fludigm will be enabling rapid growth in scientific discovery.

Join our Webinar

13:00 - 14:20Session 4 News from…Chair: Marie Burns

Chair: Marie Burns

13:00	…DRFZ: Axel Schulz: Severe COVID‐19 is characterized by an increased induction of peripheral plasmablasts with an aberrant CD62L+HLA‐DRlow phenotype (15+5)
13:20	…Munich: Selina Keppler/Marc Rosenbaum: Establishing CyTOF panels for the analysis of murine immune cells (15+5)
13:40	…Freiburg: Lena Sophie Mayer: Alterations in tissue-resident memory and exhausted-like CD8+ T cells in active UC. (15+5)
14.00	….Granada: Paulina Rybakowska: Experimental and data processing workflow for large-scale immune monitoring studies by mass cytometry (15+5)
14:20	Coffee break (20)

14:40 - 16:00Session 5 Data AnalysisChairs: Henrik Mei, Axel Schulz

Chairs: Henrik Mei, Axel Schulz

14:40	Lucie Rodriguez, Scilifelab, Stockholm, SE: Multi OMICs data integration (15+5)
15:00	Denis Schapiro, Harvard, USA: „Google Maps” for tissue biology – Mapping the tumor microenvironment with spatial omics technologies (25+5)
15:30	Mark Robinson: Differential discovery for CyTOF experiments, Univ. Zurich, CH: tba (25+5)